BIOL3380 - Biochemistry Laboratory

BIOL 3380 Biochemistry Laboratory (3 semester credit hours) Current techniques in the purification and characterization of enzymes to demonstrate fundamental principles that are utilized in modern biochemistry and molecular biology research laboratories. Practical skills taught include micropipetting, basic solution preparation, conducting pH measurements, isolating crude enzyme extracts, and performing standard activity assays. Advanced experiments with Green Fluorescent Protein and Lactate Dehydrogenase include Ni++-NTA affinity chromatography, ion chromatography, protein detection using Bradford, Lowry, and spectrophotometric assays, SDS-PAGE separation, Western Blot analysis, and enzyme kinetics. Lab fee of $30 required. A Top Hat subscription fee of up to $40 is required for this course. Prerequisite: BIOL 2281 or CHEM 2401 or equivalent. Prerequisite or Corequisite: BIOL 3461 or CHEM 3461. (1-4) S